|Technical equipment available in Proteome Center laboratories|
Flatbed system for isoelectric focusing (including IPG) and SDS-PAGE, vertical
systems for mini or large-format gels and for simultaneous runs of 12 large-format
gels, blotting cells for small and large-format gels, RotoforTM
system for preparative isoelectric focusing, Mini Whole Gel Eluter.
for computer-assisted evaluation of 2-DE gels:
Laser densitometer for scanning silver-, Coomassie blue-stained gels and films,
Kodak Image Station 2000R for colorimetric, fluorometric radiolabel and
chemiluminiscent imaging (cooled CCD camera), Melanie III software package,
SunWorkstation for storing and manipulation with gel images and another Sun
server for construction and display of 2-DE PAGE database.
Equipment for sample preparation (vacuum centrifuge, thermoblock and SymBiotTM1
sample workstation), Proteomeworks spott cutter, MALDI-TOF instrument -
Voyager DE STR, LC-MSMS instrumentation - Q-Tof UltimaTM, CapLCTM,
for purification of cell organelles:
Basic equipment for isopycnic ultracentrifugation involves GradientMaster for
pouring of sucrose gradients, bench-top ultracentrifuge Optima TM
with swinging bucket rotor and Piston Gradient Fractionater for fraction
collection, for separation of organelles based on DGE home-made electrophoresis
unit is available.
Equipment for PCR or RT-PCR analyses (electrophoresis units, cyclers and
equipment for image analysis)
microscopes Olympus BX45 and 51 and equipment for image analysis.
Fluorescence microscopes and equipment for time-lapse videomicroscopy. The
utilization of equipment for confocal microscopy and electron transmission
microscopy is based on contract with labs outside of Proteome Center.
cytometr Coulter-Epics, flow cytometer FACSCalibur