Team
 

Principal investigator and the key researchers of the Proteome center

Stulík Jiří, Assoc. Prof. M.D., Ph.D., Purkyne Military Medical Academy Hradec Kralove.

Effort (in % of full time employment ) 70
E-mail:
jstulik@pmfhk.cz

Responsible for scientific program of the center - principal investigator. Specialization - biochemistry. His laboratory work is focused on protein analysis by means of two-dimensional gel electrophoresis (2-DE). The second field of study is bioinformatics


Macela Aleš, Assoc. Prof., MSc., Ph.D., Purkyne Military Medical Academy Hradec Kralove.

Effort (in % of full time employment ) 20
E-mail:
amacela@pmfhk.cz

Specialization-cell biology and immunology. His work is aimed at monitoring of immunological parameters of host immune responses directed against intracellular bacterial pathogens. Furthermore, he is specialist in construction of in vitro and in vivo infectious models and he also works in the area of modulation of intercellular interactions of infected cells. Responsible person for postgraduate study in microbiology.


Krejsek Jan, Assoc. Prof., MSc., Ph.D., Fac. Med., Charles Univ., Hradec Kralove

Effort (in % of full time employment) 20
E-mail: ukia@fnhk.cz

Specialization-immunology. He is well-experienced in the techniques of flow cytometry, especially, in the application focused on cell immunophenotyping and intracellular measurement of cytokines. Responsible person for postgraduate study in immunology.


Červinka Miroslav, Prof., MSc., M.D., Ph.D., Fac. Med., Charles Univ., Hradec Kralove

Effort (in % of full time employment) 20
E-mail: cervinka@lfhk.cuni.cz

Specialization-cell biology. The field of his interests involves PCR techniques and special microscopy (fluorescence microscopy and time-lapse videomicroscopy)


Dostál Václav, Assoc. Prof., M.D., Ph.D., Fac. Med., Charles Univ., Hradec Kralove

Effort (in % of full time employment) 20

Specialization-infectious diseases - microbiology. He is engaged in clinical monitoring of patients and in isolation of wild bacterial strains.


Vojtěšek Bořivoj, RNDr., DSc., Masaryk Memorial Cancer Institute Brno

Effort (in % of full time employment) 20
E-mail: vojtesek@mou.cz

Specialization-molecular biology and hybridoma technology. He takes an interest in regulation of cell cycle and intracellular cell signaling. Furthermore, he is an expert in the preparation of monoclonal antibodies and production of recombinant proteins.


Sheard Michael Allen, Ph.D., Masaryk Memorial Cancer Institute Brno

Effort (in % of full time employment) 20

Specialization-cell biology. He has been performing routinely different techniques aimed at apoptosis detection (flow cytometry, Annexin-V, DNA content, M30, BrdUrd, TUNEL, MTT cell death assay)


Štětina Rudolf, RNDr., Ph.D., Purkyne Military Medical Academy Hradec Kralove.

Effort (in % of full time employment) 20
E-mail: stetina@pmfhk.cz

Specialization-toxicology - molecular biology. He is concentrated on the analysis of DNA damage and regulation of gene transcription.


Fiedler Zdeněk, M.D., Ph.D., Fac. Med., Charles Univ., Hradec Kralove

Effort (in % of full time employment) 20
E-mail: z.fiedler@lfhk.cuni.cz

Specialization-molecular biology. He will run PCR and RT-PCR techniques and, furthermore, he will study the modulation of gene expression using cDNA arrays.


Österreicher Jan, M.D., Ph.D., Purkyne Military Medical Academy Hradec Kralove

Effort (in % of full time employment) 20
E-mail: osterreicher@pmfhk.cz

Specialization-pathology, histology and radiobiology. He perform histological, histochemical and immunohistochemical analysis of tissues collected from infected model animals.


Rudolf Emil, PharmDr., Ph.D., Fac. Med., Charles Univ., Hradec Kralove

Effort (in % of full time employment) 70
E-mail: rudolf@lfhk.cuni.cz

Specialization-cell biology. He is engaged in the isolation of cellular organelles.


Hernychová Lenka, Dipl. Eng., Ph. D., Purkyne Military Medical Academy Hradec Kralove

Effort (in % of full time employment) 25
E-mail: hernychova@pmfhk.cz

Specialization-analytical chemistry. She leads mass spectrometry lab.


Kročová Zuzana, MSc., Ph.D., Purkyne Military Medical Academy Hradec Kralove

Effort (in % of full time employment) 70
E-mail: krocova@pmfhk.cz

Specialization-physical chemistry. The field of her study encompasses induction of immune response in lung after inhalation of bacterial aerosols.


Pellantová Věra, M.D., Fac. Med., Charles Univ., Hradec Kralove

Effort (in % of full time employment) 30

Specialization-infectious diseases, microbiology. Her duties include clinical monitoring of patients, isolation of wild bacterial strains and preparation of clinical samples for laboratory analyses. She is Post-Doctoral Fellow in microbiology.


Sheshko Valerie, MSc., Purkyne Military Medical Academy, Hradec Kralove

Effort (in % of full time employment) 30
E-mail: sheshko@pmfhk.cz

Specialization-molecular biology. She will be engaged in preparation of bacterial mutants harboring selectively deleted genes.


Hartmanová Blanka, MSc., Purkyne Military Medical Academy Hradec Kralove

Effort (in % of full time employment) 100
E-mail: bhartman@pmfhk.cz

Specialization-cellular and molecular biology. She is responsible for the monitoring of modulation of gene expression by infection using RT-PCR and cDNA arrays. She is PhD student in medical biology.

Hubálek Martin, MSc., Purkyne Military Medical Academy, Hradec Kralove

Effort (in % of full time employment) 70
E-mail: hubalek@pmfhk.cz

Specialization-biochemistry. He will be oriented to separation of bacterial proteins using electrophoretic and chromatographic techniques. Furthermore, he will perform structural analyses of selected proteins using LC-ESI MS/MS approach. He is PhD student in biochemistry.

Researchers and Ph.D. students of Proteome Center fulltime paid from grant budget
Andělová Hana, B.Sc.

Effort (in % of full time employment) 100
E-mail:andelovah@lfhk.cuni.cz

Specialization--medical biology and biochemistry, the analysis of biological material. She is engaged in isolation of cellular organelles and in techniques of special microscopy (confocal fluorescence microscopy and transmission and scan electron microscopy).

Černá Monika, MSc., Fac. Med., Charles Univ., Hradec Kralove

Effort (in % of full time employment) 100
E-mail: monika.cerna@seznam.cz

Specialization-clinical biology. She is responsible for establishment and maintenance of in vitro infection model. Her scientific work is aimed at modulation of in vitro microbe–host interactions by various biological response modifiers. She is PhD student in medical immunology.

Hrstka Roman, MSc., Masaryk Memorial Cancer Institute Brno

Effort (in % of full time employment) 100
E-mail: hrstka@sci.muni.cz

Specialization-molecular biology. He takes an interest in regulation of cell cycle and intracellular cell signaling. He is PhD student in molecular and cellular biology

Klimtová Ivona, PharmDr., Ph.D., Purkyne Military Medical Academy Hradec Kralove,

Effort (in % of full time employment) 100
E-mail: klimtova@pmfhk.cz

Specialization-biochemistry. The field of her study encompasses study of membrane proteins - the methods of cell fractionation, protein separation and identification.

Lenčo Juraj, MSc., Fac. Med., Charles Univ., Hradec Kralove

Effort (in % of full time employment) 100
E-mail: jurasch@seznam.cz

Specialization-molecular pathology. He focuses on specific protein expression pattern of host-pathogen interaction. He is PhD student in medical biology.

Porkertová Stanislava, MSc., Ph.D., Purkyne Military Medical Academy Hradec Kralove.

Effort (in % of full time employment) 100
E-mail: porkertova@pmfhk.cz

Specialization-immunology. She is responsible for tissue cultures and isolation of cell organelles.

Szkanderová Sylva, MSc., Purkyne Military Medical Academy Hradec Kralove

Effort (in % of full time employment) 100
E-mail: skanderova@pmfhk.cz

Specialization-clinical biology and chemistry, the analysis of biological material. She runs electrophoretic and Western blot techniques. She is PhD student in radiobiology.

Zechovská Jana, MSc., Purkyne Military Medical Academy Hradec Kralove

Effort (in % of full time employment) 100
E-mail:

Specialization-biological chemistry. She is oriented to protein separation (HPLC) and identification.
Technicians
Firychová Alena (2-DE electrophoresis, Western blotting, preparation of buffers)
Michaličková Jana (2-DE electrophoresis, Western blotting, preparation of buffers)
Lukšíková Lenka (cultivation of bacteria, MS sample preparation)
Petrová Zdeňka (Administrative supportt)
Šafek Zdeněk (Technical and logistic support)

Žáková Jitka (SDS-PAGE, work with radiolabelled samples)